Flow Cytometry

Available Instruments

 

 

Sony MA900

Excitation lasers:
488nm (7 detectors)
638nm (4 detectors)
561nm (4 detectors)
405nm (3 detectors)

Fluorochrome guide

Four way indexed sorting - numerous modes
Plate sorting
RG2 (BSLII) compliant biosafety cabinet (NSF/ANSI)

Unassisted sorting available to qualified users

*Location: rm. 420, 25 Orde street

 

Beckman Coulter MoFlo Astrios EQ, Cell Sorter

Excitation lasers:
488nm (7 detectors)
642nm (4 detectors)
561nm (4 detectors)
405nm (3 detectors)
355nm (3 detectors)

Six way indexed and mixed mode sorting
Sorting into plates (up to 1536 well) or onto slides
RG2 (BSLII) compliant biosafety cabinet (NSF/ANSI)

*Location: rm. 420, 25 Orde street

Sample preparation Tips

 
 
 

Beckman Coulter Gallios

Excitation Lasers:
488nm/561nm (5 detectors, 488nm/561nm lasers are collinear)

638nm (3 detectors)
405nm (2 detectors)

Flurochrome Guide
32 tube Multi Carousel loader (bar code capable)

Location: rm. 420, 25 Orde street and rm. 980 MSH (600 University)

 

 

 

 

Amnis ImageStreamTM Mark II (imaging flow cytometer)

Excitation lasers:
488nm (400mw), 642nm, 561nm, 592nm, 405nm
12 Imaging channels (brightfield, darkfield + 10 fluorescence)

High-Gain camera option
Multiple objectives (20X, 40X, 60X)
Autosampler (96well plate)

*IDEAS v6.3 with machine Learning module for enhanced feature extraction

Fluorochrome guide

Location: rm. 980 MSH

 

 

 Fortessa HTS

BD Fortessa X-20 with HTS

Excitation Lasers
355nm, 405nm, 488nm, 561nm, 640nm 
18 colors (UV-2/V-6/B-2/Y-5/R-3)
Configuration

High Through-put Sampler (HTS) 96/384well plate

Location: rm.980 MSH

 

 

Analysis Workstation with Tesla GPU accelerator card and 30in monitor

Software

Kaluza analysis software

FlowJo version X

ModFit LT

 
 
 

Attune NxT/CytKick Max autosampler

Excitation Lasers
405nm,488nm,561nm,638nm
14 Colors - (V6/B2/Y3/R3)
High Throughput autosampler 96/384 well + 24 X 1.5ml microfuge tube rack with cooling

Fluorochrome guide

 

 

          
 

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